ALEXANDRIA, Va., March 17 -- United States Patent no. 12,578,341, issued on March 17, was assigned to UNIVERSITAT ZURICH (Zurich, Switzerland).
"Iterative fluorescence imaging" was invented by Gabriele Gut (Zurich, Switzerland), Lucas Pelkmans (Zurich) and Markus Herrmann (Boston).
According to the abstract* released by the U.S. Patent & Trademark Office: "The invention relates to a method for multiplex staining of a biological sample involving the use of a buffer combination of blocking buffer, imaging buffer and elution buffer that allows for multiple staining rounds of biological samples. The blocking buffer comprises a compound that is capable of binding to hydrophobic binding sites non-specifically and a sulfhydryl-reactive compound....