ALEXANDRIA, Va., March 17 -- United States Patent no. 12,577,559, issued on March 17, was assigned to Di Wu (Solna, Sweden).
"Method for creating a cDNA sequencing library" was invented by Di Wu (Solna, Sweden), Tomasz Wirt (Enskede, Sweden) and Mats Nilsson Bernitz (Drottningholm, Sweden).
According to the abstract* released by the U.S. Patent & Trademark Office: "The present invention relates to the field of biotechnology and a method for creating a cDNA library. More specifically, the invention refers to a method of forming complementary DNA (cDNA) sequencing libraries from RNA in situ comprising the steps of: (a) fixating cells, immobilized on a solid surface; (b) performing an in situ reverse transcription (RT) inside cells, using RT...