US Patent Issued to Sarepta Therapeutics on March 17 for "Suspension mode seed train development for adherent cells" (Massachusetts Inventor)

ALEXANDRIA, Va., March 17 -- United States Patent no. 12,577,540, issued on March 17, was assigned to Sarepta Therapeutics Inc. (Cambridge, Mass.).

"Suspension mode seed train development for adherent cells" was invented by Maroof Alam (Cambridge, Mass.).

According to the abstract* released by the U.S. Patent & Trademark Office: "The disclosure is directed to a method for seed train expansion of adherent cells comprising culturing cells with a serum-supplemented growth medium in a N-2 vessel; removing the cells from the serum-supplemented medium; inoculating the cells from step into a serum-free growth medium in a N-1 vessel; culturing the cells in the N-1 vessel under suspension conditions; and inoculating a growth medium in a bioreactor...