US Patent Issued to Massachusetts Institute of Technology on Jan. 20 for "Applications of recombined ScCas9 enzymes for PAM-free DNA modification" (Massachusetts Inventors)

ALEXANDRIA, Va., Jan. 20 -- United States Patent no. 12,529,042, issued on Jan. 20, was assigned to Massachusetts Institute of Technology (Cambridge, Mass.).

"Applications of recombined ScCas9 enzymes for PAM-free DNA modification" was invented by Pranam Chatterjee (Cambridge, Mass.) and Joseph M. Jacobson (Newton, Mass.).

According to the abstract* released by the U.S. Patent & Trademark Office: "SpRYc is a grafted ScCas9++-SpRY chimeric Cas9 possessing minimal 5'-NNN-3' PAM specificity. SpRYc comprises the N-terminus (residues 1-1119) of ScCas9++ (Sc++), including the flexible loop, followed by the region of SpRY (residues 1111-1368) spanning its PAM-interacting domain mutations. Methods of altering gene expression include use of SpRYc ...