US Patent Issued to Insitro on June 30 for "In situ sequencing of RNA transcripts with non-uniform 5' ends" (California Inventors)

ALEXANDRIA, Va., July 16 -- United States Patent no. 12,668,841, issued on June 30, was assigned to Insitro Inc. (South San Francisco, Calif.).

"In situ sequencing of RNA transcripts with non-uniform 5' ends" was invented by Cynthia Hao (Saratoga, Calif.), Max R. Salick (San Francisco) and Ci Chu (Palo Alto, Calif.).

According to the abstract* released by the U.S. Patent & Trademark Office: "Disclosed herein are methods for performing in situ sequencing of RNA transcripts with non-uniform 5' ends. During reverse transcription (RT) of RNA transcripts, RT enzyme is induced to "template-switch" to a separate oligonucleotide provided as the template for the upstream flanking region. This flanking region is grafted onto the beginning of the cD...