US Patent Issued to IDENTIGEN on March 17 for "Multiplex method of preparing a sequencing library" (Idaho Inventor)

ALEXANDRIA, Va., March 17 -- United States Patent no. 12,577,616, issued on March 17, was assigned to IDENTIGEN Ltd. (Dublin).

"Multiplex method of preparing a sequencing library" was invented by Nathan Campbell (Twin Falls, Idaho).

According to the abstract* released by the U.S. Patent & Trademark Office: "A method of preparing a library of library constructs by multiplex amplification for use in targeted next generation sequencing is described. The method comprises the steps of: (a) providing a reaction vessel comprising (i) a plurality of different target sequences, (ii) a plurality of target capture primer pairs, and (iii) one or more tagging primer pairs, (b) performing sequential rounds of amplification at sequential annealing tempe...