ALEXANDRIA, Va., April 7 -- United States Patent no. 12,596,120, issued on April 7, was assigned to Hoffmann-La Roche Inc. (Little Falls, N.J.).
"PH-gradient SPR-based binding assay" was invented by Tilman Schlothauer (Penzberg, Germany) and Christian Spick (Penzberg, Germany).
According to the abstract* released by the U.S. Patent & Trademark Office: "Herein is reported a method for determining the dissociation rate constant kd of an antibody from its antigen at the dissociation pH-value, wherein the method comprises the steps of immobilizing at a first pH-value the antibody on a solid phase to which the antigen of the antibody has been conjugated; applying a pH-gradient from the first pH-value to the dissociation pH-value and thereafter...